Three seeds advanced on Day 2 at Tashkent, while two seeds including local favorite No. 7 seed Akgul Amanmuradova fell in disappointing straight sets.
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No. 2 seed Olga Govortsova was the other disappointed seed, as she fell in three sets to Ksenia Palkina of Kyrgyzstan, 76(5) 46 75.
"I'm really stunned with my win, I went to the court to win and I believed in myself," said Palkina, who is currently ranked No.203.
"At 1-3 in the third set I felt that I would lose, but the crowds cheered for me and I knew I had to win and it all changed from that moment. It's my first upset on the WTA Tour and at my favorite event too," she added.
Amanmuradova lost shamefully among her home crowd, to Urzsula Radwanska, last year's junior Wimbledon champion, 76(5) 63.
"I'm frank in telling you that I could not handle the pressure of my country people, I had my chances at 3-1 in the tie-break, but could not take it," the 24-year-old Amanmuradova said. "I'm angry at myself; at this level it is a crime to lose such an advantage."
"It's always difficult to play a star in their country, but I've watched Akgul playing Agnieszka in Rome earlier, and I'd made a plan to play her," Radwanska said. The Polish teen said breaking Amanmuradova's difficult serve in the very first game made it easier for her to handle it for the rest of the match.
Top seed Shuai Peng defeated Arina Rodionova, No. 3 seed Sorana Cirstea defeated Ekaterina Dzehalevich, 76(4) 75, and No. 6 seed Monica Niculescu advanced after winning the first set and having Olga Savchuk retire early in the second set, 75 01.